Objective To investigate the clinical value of 320-row dynamic volume CT coronary angiography in radiofrequency ablation for atrial fibrillation in. Methods 60 cases of patients with atrial fibrillation in our hospital and 40 healthy subjects (the control group) were as the research object; they were used 320-row dynamic volume CT coronary angiography and abnormal enhancement of left atrial thrombus in the diagnosis of pulmonary vein. Results There were 23 cases (38.3%) of variation of pulmonary in atrial fibrillation group, while 17 (34.0%) cases in the control group. The variation of pulmonary vein in the AF group and the control group were no statistically significant in different gender and different parts (P>0.05). The left and right upper pulmonary vein long diameter and short diameter, left and right, vein under long diameter and short diameter in the AF group was longer than that in the control group, the difference was statistically significant (P<0.05). Left atrial thrombus diagnosis rate by 320-Row and echocardiography was no statistically significant (P>0.05). In 60 cases of patients with atrial fibrillation，42 patients had coronary plaque lesions lumen which showed mildsevere stenosis. Conclusion 320-row dynamic volume CT coronary angiography can diagnosis pulmonary venous anomalies well in atrial fibrillation patients, which can be used as a routine imaging before atrial fibrillation ablation imaging method.