High-throughput Sequencing Analysis of Intestinal Microbiota in Patients with Irritable Bowel Syndrome and Healthy Individuals*
Author：YANG Li-li, WANG Cheng-wen,ZOU Ao
affiliation：Shantou University Medical College, Shantou 515041, Guangdong Province, China
PDFAbstract
Objective To compare differences of intestinal microbiotabetween IBS patients andhealthy by using high-throughput sequencing technology. Methods The feces were collected IBS group andhealthy group. The community structure and abundance of intestinal microbiota were examined via analyzing 16S rRNA high throughput sequencing using Illumima System. Results The diversities of intestinal bacteria were no significant differences in IBS group. In phyla level, the abundance of Firmicutes was significantlyincreased (P<0.01), while the abundance of Bacteroidetesand the ratio ofBacteroidetes/Firmicutes were decreased (P<0.01). Besides, in family level, cryomorphaceae,erysipelotrichac eae. Campylobacteraceae and fibrobacteraceae were detected in IBS group and healthy group.Their abundance were significantly difference, respectively (P<0.05). Conclusion No significantdifferences in the bacterial diversity indices are found, but some bacterial species significantly differbetween the patients with IBS and healthy controls.
【Keyword】 Irritable Bowel Syndrome; Intestinal Microbiota; High-throughput Sequencing; 16S rRNA
【Chart number】R378.2
【Document Identification Number】A
【DOI】10.3969/j.issn.1009-3257.2017.05.015
Journal of Rare Diseases
th24Volume， th 5 Issue
2019Year11Month
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