Objective To study the expression level and clinical significance of interleukin-6 (IL-6) in pregnant women with premature rupture of membranes. Methods From Jan. 2015 to Feb. 2016, a total of 70 cesarean section pregnant women with premature rupture of membranes in our hospital were selected as observation group, at the same time another 29 normal pregnant women were selected as control group. The IL-6 levels in the two groups were compared. Results The serum IL-6 level in the observation group was (0.71±0.12) µg/L which was significantly higher than that in the control group (0.42±0.09)µg/L(P<0.05), the serum IL-6 level in the mature group was (0.64±0.10) µg/L which was obviously lower than that in the premature group (0.81±0.15) µg/L (P<0.05), the level of serum IL-6 in the infected group was significantly higher than that in the uninfected group (P<0.05), the level of serum IL-6 in the severe infected patients was significantly higher than that in the mild and moderate infected patients(P<0.05), the serum IL-6 level in the moderate infected patients was significantly higher than in the mild infected patients(P<0.05). Conclusion The expression level of serum IL-6 is a better indicator for detecting the chorioamnionitis of premature rupture of membranes.