Objective To explore the effects and significance on platelet antibody detection and platelet transfusion effect of patients with lymphoma. Methods 50 patients from September 2013 to September 2015 with lymphoma in our hospital were selected as research subjects. Firstly, patient′ times of transfusion were counted and their platelet antibody was tested before and after the transfusion, then observe the relation between the positive rate of platelet antibody and times of transfusion, afterwards, the patients received platelet transfusion and the test result of platelet antibody and its relation with the effect of platelet transfusion were evaluated; at last, the 50 patients were randomly allocated to test group and control group, with 25 patients in each group. Patients in the control group received random platelet transfusion and patients in the test group received matched platelet transfusion. The effect of platelet transfusion between the two groups was evaluated. Results 23 cases(46.0%)of 70 patients had positive test result of platelet antibody. With the times of transfusion increasing, the positive rate of platelet antibody gradually increases(P<0.05). The PTR in positive platelet antibody was 60.87%,the PTR in negative platelet antibody was 29.63%,with statistical difference (P<0.05).The effective rate of platelet transfusion in control group was 40.0%,and that in test group was 72.0%,with statistical difference(P<0.05). Conclusion More of the times of blood transfusion will increase more platelet antibody, platelet antibody detection and matched platelet transfusion of patients with lymphoma. have great significance for improving the effects of platelet transfusion.