Objective To investigate the application value of quality control circle activity in improving neonatal nursing knowledge awareness rate for primipara. Methods From Feb. 2014 to Feb. 2015, a total of 110 primiparas in our hospital were taken as the clinical research objects, and they were divided into observation group (55 cases) and control group(55 cases). Only the patients in the observation group participated in the theme activity “improving neonatal nursing knowledge awareness rate for primipara”.The clinical data in the two groups were analyzed retrospectively, and the neonatal nursing knowledge awareness rates for primipara in the two groups were also compared. Results Before nursing, the observation group awareness rate was 42%, the control group awareness rate was 50%, and the difference was not statistically significant (P>0.05). After nursing, the observation group awareness rate was higher than that of control group (76% vs 58%), the difference was statistically significant (P<0.05). The nursing ability of observation group was higher than that of control group (98.18% vs 65.45%), the difference was statistically significant (P<0.05). Conclusion Quality control circle activity which has obvious effect on improving neonatal nursing knowledge awareness rate for primipara, can achieve good result in clinic. Thus, it is worthy of clinical application.