目的 探索1 H-MRS活体检测结肠 癌耐药性的可能性。方法 5只耐药(耐 5-FU)与5只不耐药人类结肠癌SW480荷 瘤裸鼠于肿瘤直径大于1.5cm时行1 H-MRS 检查，随后处死荷瘤鼠，切下肿瘤检测 其PKC、P-gp、MRP1蛋白表达(Western Blot)，观察肿瘤凋亡(TUNNEL法)。分析 结肠癌耐药性与1 H-MRS检测代谢物、肿瘤 耐药基因表达、肿瘤凋亡的相关性。结 果 不耐药组人类结肠癌SW480裸鼠肿瘤 的cho、lac、Lip及mI峰/cr面积比较耐 药组人类结肠癌SW480/5-FU裸鼠增加， 差别有统计学差异(P＜0.05)。耐药组 PKC、P-gp、MRP1蛋白表达明显高于不耐 药组，差别有统计学差异(P＜0.05)。在 TUNNEL检测切片上，两组肿瘤组织均可见 肿瘤细胞凋亡，两者差别无统计意义(P＞ 0.05)。肿瘤代谢物cho、Lac、Lip、mI/ cr与肿瘤耐药性呈正相关性；cho面积与 肿瘤PKC表达光密度呈正相关，与肿瘤Pgp表达光密度呈正相关。结论 肿瘤代谢 物cho、lac、Lip的改变有可能有助于利 用1 H-MRS活体检测结肠癌耐药性改变。

Objective To exploration the feasibility to detect the drug resistance of human colon cancer in mice with magnetic resonance spectrum in vivo. Methods Nude mice with human colon cancer SW480(drug response) and 5 nude mice with SW480/5-Fu(5- Fu resistance) were given 1 H-MRS examinations when the diameters of tumor were over 1.5 centimeters. Then these mouse models were sacrificed. Their tumors were removed and detected the protein expression of PKC, P-gp and MPR1 (Western blot), apoptosis (TUNNEL). The correlation between metabolites detected with 1 H-MRS and tumor drug resistance and the protein expression of PKC, P-gp and MPR1 (Western blot), tumor apoptosis were analyized. Results The the area of cho, lac and Lip of tumors in SW480 group were significantly higher than that in SW480/5-Fu group (P＜0.05). The protein expression of PKC, P-gp and MPR1 in SW480/5-Fu group were significantly higher than that of SW480 group (P＜0.05). In the TUNNEL section, apoptotic cell were observed in two groups, and there is no significant difference between two groups (P＞0.05). The area of cho, lac and Lip of tumors was positively correlated with drug resistance (r, P:0.870,0.001). The the area of cho has a positive correlation with the protein expression of PKC (r, P:0.745,0.013), P-gp (r, P:0.782,0.008). Conclusion The change of metabolites cho, lac and Lip detected with 1 H - MRS has the potential to help detection of colon cancer drug resistance with MR in vivo.