Objective To study and analyze the clinical effect of CPAP ventilator combined with ambroxol in neonatal respiratory distress syndrome. Methods One hundred newborns with respiratory distress syndrome were selected from February 2014 to February 2016. The patients were divided into two groups according to the order of treatment. One group was treated with conventional mechanical ventilation combined with ambroxol Control group, one group was treated with CPAP ventilator combined with ambroxol observation group, each group of 50 cases, the observation of two groups of children in the treatment of effective blood gas before and after treatment indicators. Results The effective rate of treatment was 98% (49/50) in the observation group and 84% (42/50) in the control group. There was significant difference between the two groups (χ2 =7.111, (P<0.05). The blood gas index of the observation group was significantly better than that of the control group. After 24h and 48h, the PaO2 of the observation group and the control group increased significantly, PaO2 decreased, FiO2 decreased, OI decreased, Compared to the difference was significant. Conclusion Neonatal respiratory distress syndrome with CPAP ventilator combined with ambroxol treatment, the effect is obvious, effective treatment of high blood gas recovery index is good, has a very high promotion value, should be vigorously promoted.