Optimization of IFITMs Primers in Respiratory Tract infection Samples
Author：ZUO Qing-li, LU Yan-qin, HAN Jin-xiang
affiliation：University of Jinan-Shandong Academy of Medical Sciences, Jinan 250200, Shandong Province, China
PDFAbstract
Objective To optimize the five subtypes of interferon inducible transmembrane proteins, namely IFITM1, IFITM2, IFITM3, IFITM5 and IFITM10. Methods Extraction of nucleic acids from respiratory tract infection samples. Specific primers were screened by qRT-PCR and Ct value was between (15,35), and the specificity of primers was verified by agarose gel electrophoresis. Results IFITM1-3 was specific in the IFITM1 primers. IFITM 2-2 was specific in the IFITM2 primers. IFITM3-2 was specific in the IFITM3 primers. IFITM5-6 was specific in the IFITM5 primers. IFITM10-1, IFITM10-2 and IFITM10-3 were specific in the IFITM10 primers. Agarose gel electrophoresis bands of all primers with specificity were single bright. Conclusion IFITM1-3 was superior to other primers of IFITM1 primers. IFITM 2-2 was superior to other primers of IFITM2 primers. IFITM3-2 was superior to other primers of IFITM3 primers. IFITM5-4 was superior to other primers of IFITM5 primers. IFITM10-1, IFITM10-2 and IFITM10-3 were superior to other primers of IFITM10 primers.
【Keyword】Respiratory Tract Infection; Primer; IFITMs
【Chart number】R373.1
【Document Identification Number】A
【DOI】10.3969/j.issn.1009-3257.2017.02.009
Journal of Rare Diseases
th24Volume， th 2 Issue
2019Year11Month
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