Objective To study the correlation of the Hcy (homocysteine) level with arteriosclerosis-associated cerebral ischemic stroke. Methods 58 patients with arteriosclerosis-associated cerebral ischemic stroke treated from March 2014 to March 2017 in our hospital were selected (observation group). 58 healthy check-ups in our medical examination center were selected (control group). The index of Hcy, FBG and blood lipid for two groups were observed; the correlation of Hcy with the arteriosclerosis-associated cerebral ischemic stroke was analyzed. Results The index of Hcy for observation group was significantly higher than control group (P<0.05); the index of FBG, TC, TG, LDL-C and HDL-C for two groups were not significantly different (P>0.05), the index of Hcy had a close correlation with the arteriosclerosis-associated cerebral ischemic stroke(P<0.05); the other index (FBG, blood lipid, etc) had no obvious correlation with the arteriosclerosis-associated cerebral ischemic stroke (P>0.05). Conclusion The index of Hcy for arteriosclerosis-associated cerebral ischemic stroke patients is significantly higher than healthy group; the higher the Hcy level is, the more serious the illness condition is.