Objective To explore the nursing cooperation of ultrasound-guided histological biopsy. Methods 63 patients who underwent ultrasound-guided histological biopsy from November 20, 2017 to December 20, 2018 in our hospital were selected for the study and were divided into observation group (33 cases) and control group (30 cases) according to the random number table method. Control group was given routine treatment measures, and observation group was given nursing cooperation on the basis of control group. The pain visual analogue score (VAS), self-rating anxiety scale(SAS) and self-rating depression scale(SDS) were used to evaluate the biliousness and negative emotions in the two groups. The completion of puncture and the success of puncture and occurrence of complications were compared between the two groups. Results The scores of VAS, SAS and SDS in observation group were significantly lower than those in control group(P<0.05). The puncture completion rate and puncture success rate were 84.85% and 60.00% in observation group, which were significantly higher than those in control group with 75.76% and 40.00%(P<0.05). The incidence rate of complications in observation group was significantly lower than that in control group(6.06% vs 26.67%) (P<0.05). Conclusion Ultrasound-guided histological biopsy can improve negative emotions, relieve biliousness, improve puncture success rate and reduce occurrence of complications.