Objective To analyze the effect of the first mobile blood donation house in Zhaoqing city by comparing the number of blood donors before and after the use of mobile blood donation house in dalufa fixed blood donation point in Zhaoqing city. Methods The number of unpaid and mutual-aid blood donors in dareunfa fixed blood donation sites in Zhaoqing city in January 2017 and January 2018 was queried by Maike Blood System. SPSS 18.0 software was used for statistical analysis. Results In January 2017, 370 people donated blood (333 people donated blood without compensation, 37 people donated blood with mutual assistance), and in January 2018, 588 people donated blood (552 people donated blood without compensation, 36 people donated blood with mutual assistance). The number of free (or mutual) blood donors in January 2017 and January 2018 was compared, and the difference was statistically significant (chi-square =4.85, P=0.027).333/37, 552/36). Conclusion The environment of blood donation in mobile blood donation house is better than that in general blood donation vehicle, which is easier for donors to accept. Improving the environment of blood donation is very important to increase the number of blood donors.