Objective To explore the effects of palliative care on cancer-related fatigue and negative emotions in patients with advanced pancreatic cancer. Methods A total of 102 patients with advanced pancreatic cancer admitted to our hospital from December 2015 to December 2017 were selected for the study and were divided into experimental group and control group according to the lottery method, with 51 cases in each group. Control group was given routine care, and experimental group was given palliative care measure on the basis of control group. The subjective fatigue discomfort and negative emotions were evaluated by PIPER-Fatigue Scale (PFS), Hamilton Anxiety Scale (HAMA) and Hamilton Depression Scale (HAMD) before nursing intervention and after 1 month of nursing intervention. Results After 1 month of nursing intervention, the moderate-severe fatigue rate and scores of HAMA and HAMD in experimental group were significantly lower than those before intervention, and were lower than those in control group at the same time period (all P<0.05). There were no significant changes in the moderate-severe fatigue rate and scores of HAMA and HAMD in control group before and after nursing intervention (all P>0.05). Conclusion Palliative care can improve the cancer-related fatigue and negative emotions in patients with advanced pancreatic cancer.