Objective To analyze the application and nursing measures of high frequency oscillatory ventilation (HFOV) in neonates with pulmonary hemorrhage. Methods 30 neonates with pulmonary hemorrhage admitted to our hospital were selected for the study and randomly divided into high frequency group and routine group, with 15 cases in each group. Routine group was treated with constant frequency mechanical ventilation, and high frequency group was treated with HFOV. The clinical indexes of pulmonary hemorrhage time, hospital stay and ventilator operation time, blood gas indexes of partial pressure of carbon dioxide (PaCO2) and partial pressure of oxygen (PaO2) before and after treatment and the incidence rate of complications were compared between the two groups. Results The pulmonary hemorrhage time, hospital stay and ventilator operation time in high frequency group were shorter than those in routine group (P<0.05). After treatment, the PaCO2 level in high frequency group was lower than that in routine group while the PaO2 level was higher than that in routine group (P<0.05). The incidence rate of complications was 26.67% in high frequency group and 40.00% in routine group (P>0.05). Conclusion HFOV combined with reasonable nursing measures for neonatal pulmonary hemorrhage can not only significantly shorten the pulmonary hemorrhage time, hospital stay and ventilator operation time, but also improve blood gas indexes, and it has no significant impact on complications