Objective To investigate the influence of nursing intervention on psychological status and agitation in patients undergoing general anesthesia during awakening period, aiming to provide reference for future clinical nursing. Methods Eighty patients with general anesthesia in our hospital from July 2016 to September 2017 were enrolled in this study. The patients were divided into two groups by random number table. The control group was given routine nursing intervention. The observation group was given operation nursing intervention , And observed the two groups of patients with psychological conditions and restlessness. Results The scores of anxiety after anesthesia in both groups were significantly lower than those before anesthesia (P<0.05). After anesthesia, the anxiety scores in the observation group were significantly lower than those in the control group (P<0.05), and the recovery time was significantly shorter than that in the control group.The incidence of agitation in observation group (15.00%) was significantly lower than that in control group (50.00%), P<0.05 by χ2 test. Conclusion The implementation of operating room nursing intervention in patients with general anesthesia during awakening can effectively eliminate the adverse psychological conditions and reduce the occurrence of agitation, which is worthy of clinical application and promotion.