Objective To observe the imaging findings of contrast-enhanced ultrasound and computed tomography (CT) in splenic tuberculosis, and explore the value of two imaging methods in the diagnosis of splenic tuberculosis. Methods From January 2009 to March 2014, the clinical data of 26 patients with splenic tuberculosis confirmed by surgery and pathology in outpatient department in our hospital were retrospectively analyzed. All imaging findings of contrast-enhanced ultrasound and CT were analyzed and compared, and the application value of the two diagnostic methods was evaluated. Results Contrastenhanced ultrasound showed low echo type, mixed echo type and strong echo type, and the findings were basically the same, and without enhanced type was the most common finding of contrast-enhanced ultrasound. CT scan showed multiple different sizes of nodular slightly low density in spleen, and the boundaries were not clear, while the enhanced CT scan showed that boundaries and internal separation obviously were not enhanced, and compared with surrounding spleen, there was significantly low-density shadow, similar signs of cystic lesions and calcification were relatively typical manifestations of splenic tuberculosis. There was no significant difference in accurate rate between contrast-enhanced ultrasound and CT in the diagnosis of splenic tuberculosis (P>0.05). Conclusion Both of contrast-enhanced ultrasound and CT have specificity in the diagnosis of splenic tuberculosis, and are worthy of clinical application.