Objective To research the application value of 3.0T magnetic resonance diffusion tensor imaging in the central zone lesions of prostate. Methods Ten healthy volunteers, 15 CG-PCa cases and 20 BPH cases proven by histopathology were performed MRI and DTI. ADC values and FA values of CG-PCa and BPH were respectively measured, comparing whether there is any discrepancies among the three groups. For DTT maps, two observers recorded the score using a four-point scale and compared the differences of fibers continuity and density in the three groups. Results ADC values and FA values in CZ-PCa were: (1026±176)×10-6mm2 /s, (0.46±0.02)×10-1. BPH were(1351±143)×10-6mm2 /s, (0.23±0.04)×10-1, healthy volunteers were (1458±188)×10-6mm2 /s, (0.22±0.05)×10-1. ADC value of the three groups were statistically different(P<0.05)while FA values was not(P>0.05). The DTT map score of CZ-PCa were (2.8±0.9), (2.9±0.4), the BPH were (1.6±0.8), (1.7±0.42), healthy volunteers were T(1±0.2), (1±0.3). The consistency score of the three groups by two observers was good (k=0.723). The DTT map score between the three groups for two viewers were all significantly different(P<0.05). Conclusion ADC values can play a significant role in the differentiation of CG-PCa and BPH. DTT maps can show prostate fibers more Intuitively and it is helpful in differentiating PCa from BPH.