Objective To explore the value of 64-slice dynamic volume CT used for assessment of coronary artery stenosis and the correlation of cardiac function of left ventricular. Methods Seventy seven patients with coronary artery stenosis were selected as research subjects, and they were divided into the observation group, 28 candidates undergoing healthy physical examinations without coronary artery stenosis were selected into the control group for research. All subjects underwent 64-slice dynamic volume CT and two-dimensional echocardiography on left ventricular function, and left ventricular function parameters of two groups of patients were analyzed respectively by use of two methods. Results ESV value, EDV value, SV value and EF value measured by 64-slice & 64-lamina dynamic volume CT with two-dimensional echocardiography via Peasorn correlation inspection showed good consistency (r=0.72-0.85). EDV, ESV, EF and MM values were significantly and positively correlated to the degree of coronary artery stenosis (r=0.21, 0.45, 0.49, 0.45, P<0.05). There was no significant difference between groups in SV value, and the extent of coronary stenosis was of no correlation (r=0.07, P>0.07). Conclusion 64-slice dynamic volume CT delivers high feasibility in simultaneously assessing functions of coronary artery stenosis and left ventricular, this technique can accurately the damage of evaluate coronary stenosis on cardiac function of left ventricular, greater damage of left ventricular function will be when the degree of coronary artery stenosis is heavier, providing important basis for clinical treatment