Objective To explore the correlation between multislice computed tomography (MSCT) features and angiogenesis in hepatocellular carcinoma (HCC). Methods Fortyone cases of surgically excised HCC tissue samples that were pathologically diagnosed were analyzed by Elivisonimmunohistochemistry to detect the expression of hypoxiainducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) and assess microvessel density (MVD). All of the patients were preoperatively examined with plain and enhanced CT. Results The differences of positive expression rates of HIF-1α and VEGF and the MVD values in HCC with a tumor diameter ≤ 5cm and >5 cm were not statistically significant (P>0.05). The positive expression rates of HIF-1α and VEGF and the MVD values in tumors with ruptured pseudocapsules or without pseudocapsules, a high risk of metastasis and invasion, and arterial blood were higher than those in tumors with intact capsules, a low risk of metastasis and invasion, and double blood supply, the group differences were statistically significant(P<0.05). Conclusion MSCT manifestations of HCC with HIF-1 alpha, closely related to the expression of VEGF and MVD value; MSCT signs can reflect HCC pathological and biological characteristics.