Objective To explore and compare the value of Doppler ultrasonography and multi-slice spiral CT in the differential diagnosis of solid pseudopapillary tumor of pancreas (SPTP). Methods The ultrasonic and CT findings of 26 patients with SPTP diagnosed in our hospital from March 2012 to Match 2016 were retrospectively analyzed. All findings were analyzed and compared with the results of operation and pathology. Results The ultrasonic findings showed 22 cases with complete capsule and clear boundaries, and 6 cases with calcified tissues. The cystic solid component was the main part, and the solid part showed homogeneous low echo while the cystic part showed low or no echo area. Most CDFI showed punctiform blood flow signal in the echo area. CT scan was low density, and there were 22 cases with complete capsule and 10 cases with patchy or ring calcified tissues. The cystic components were the majority, solid part showed papillary structure. In venous phase, tumor parenchyma showed obvious enhancement, in portal venous phase and delayed phase, it showed further enhancement, with progressive features, which was still lower than the solid parts of normal pancreas. The cystic part was dispersed in the solid part, and no obvious enhancement was observed. There were no signs of pancreaticobiliary duct dilatation and lymph node metastasis. The diagnostic accuracy rate of CT (84.62%) was higher than that of ultrasound (P<0.05). Conclusion The ultrasonic and CT imaging of SPTP are characteristic, which can be combined with clinic for differential diagnosis of other diseases. The accuracy rate of preoperative diagnosis of CT is higher than ultrasonography.