Objective To investigate the application value of transvaginal three dimensional ultrasonic salpingography in the diagnosis of infertility. Methods 50 cases with infertility in our hospital from January 2016 to December 2016 were selected as study objects, they were randomly divided into two groups according to number table method, 25 cases in the control group were given two dimension HSUG inspection, 25 cases in the observation group was given transvaginal three dimensional ultrasonic salpingography inspection, observe the results of inspection, compare the inspection results with operation pathological results. Results Clinical coincidence rate of the observation group and the control group were 96.00%, 68.00%, the difference was statistic significant (P<0.05), the probability of tubal patency, tubal partially obstructed, tubal obstruction of the observation group and the control group were 32.00%, 28.00%, 36.00% and 32.00%, 32.00%, 36.00% respectively, the difference was not statistic significant (P>0.05). Conclusion The transvaginal three dimensional ultrasonic salpingography has significant clinical value in the diagnosis of infertility, it can lower rate of missed diagnosis, improve diagnostic rate of patients, it is worthy of popularization and application.