Objective To investigate and analyze the diet and exercise intervention on blood glucose during pregnancy in pregnant women with diabetes mellitus control. Methods Selected in our hospital during January 2015-2016 year in December 242 cases of gestational diabetes in pregnant women as research subjects, according to the sequence of women were divided into group a and group B, each of the 121 cases of pregnant women B, two groups of pregnant women were given routine nursing care, the measures for pregnant women with diet and exercise intervention, group B pregnant women treated with short acting insulin, a comparison of two groups of pregnant women blood glucose levels, maternal complications and cesarean section rate. Results The group of pregnant women fasting blood glucose levels, 2h postprandial blood glucose was higher than B but the difference between groups of pregnant women, there was no statistical significance (P>0.05). The incidence of maternal complications and cesarean section rate were higher than those in group B, but there were no statistical differences between Significance (P>0.05). Conclusion In the nursing care of pregnant women with gestational diabetes, the application effect of diet and exercise intervention is very good, quite the same injection of short acting insulin effect, effectively improve maternal blood glucose levels, and reduce the risk of maternal complications, reduce the cesarean section rate, worthy of promotion.