Objective To study the effect of oxytocin on blood gas analysis of fetal cord blood. Methods From Oct. 2016 to Mar. 2017, a total of 72 pregnant women in our hospital were taken as the clinical objects, which were randomly divided into A group (36 cases) and B group (36 cases). In the process of delivery, only A group was given oxytocin. The blood gas analysis of fetal cord blood in the two groups was compared. Results The blood glucose, blood chlorine, blood sodium and pH value in the A group were respectively(2.14±0.20)mmol/L,(92.13±3.14)mmol/L,(130.27±3.42) mmol/L and(7.09±0.08) which were obviously lower than those in the B group(2.63±0.38)mmol/L,(98.72±1.73)mmol/ L,(139.28±3.97)mmol/L and (7.28±0.07)(P<0.05), the bilirubin and BE in the A group were respectively(28.36±3.75) mg/L and(0.28±0.11)mmol/L which were obviously higher than those in the B group (12.64±2.13)mg/L and (0.14±0.05) mmol/L. The incidence rate of neonatal hyperbilirubinemia in the A group was 63.89% which was obviously higher than that in the B group 33.33%(P<0.05). Conclusion In the process of delivery, intravenous oxytocin can cause neonatal hypoglycemia, hyponatremia or hypochloraemia, reduce the blood pH value, raise the level of bilirubin and BE, increase the risk of neonatal hyperbilirubinemia and acidosis. Therefore, oxytocin should be rationally applied in clinic.