Objective To share the nursing experience of 5S nursing mode for patients after complex anal fistula operation. Methods From Apr. 2013 to Jan. 2016, a total of 74 patients accepted complex anal fistula operation in our hospital were taken as the research objects, and they were divided into observation group (n=37) and control group (n=37) according to their visiting orders. The observation group was given 5S nursing mode, and the control group was given routine nursing for anal fistula operation. The nursing experiences of two different nursing modes were compared. Results The hospitalization time, wound healing time, inpatient experience questionnaire (IPEQ) score, self rating anxiety and depression scale scores in the observation group were significantly lower than those in the control group; the adverse reaction rate in the observation group during hospitalization was 8.10% which was lower than that in the control group 27%, the differences between the two groups were statistically significant (P<0.05). Conclusion 5S nursing mode which is beneficial to the recovery of postoperative patients with complex anal fistula operation, can not only improve patients' experience in hospital, but also reduce the stress effects of anxiety, depression and other adverse reactions.