Objective To observe the effects of continuous blood purification (CBP) on intestinal barrier function in patients with severe acute pancreatitis (SAP). Methods 60 patients with SAP admitted to our hospital were selected for the study and were randomly divided into CBP treatment group and control group, with 30 cases in each group. Control group was given routine treatment, and CBP treatment group was given CBP treatment on the basis of control group, and the two groups were treated for 1 w. The clinical efficacy of the two groups was compared. The levels of diamine oxidase (DAO), D-lactic acid and endotoxin were measured by spectrophotometry. The intestinal barrier function of the two groups was compared. The levels of inflammatory factors of serum tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-8 (IL-8) were detected by enzyme-linked immunosorbent assay. Results The total effective rate of treatment was 90.00% in CBP treatment group and 83.33% in control group (P<0.05). After treatment, the levels of DAO, D-lactic acid and endotoxin and the levels of inflammatory factors in the two groups were decreased, and the levels of DAO, D-lactic acid, endotoxin and TNF-α, IL-6 and IL-8 in CBP treatment group were lower than those in control group (P<0.05). Conclusion CBP can improve the intestinal barrier function by reducing the levels of inflammatory factors in SAP patients, and it can significantly improve the efficacy of SAP, and it has certain clinical significance.