Objective To explore the application value of multi-slice spiral CT (MSCT) in the localization diagnosis of embedded teeth. Methods The clinical data of 76 patients with embedded teeth who were treated in department of stomatology of our hospital from May 2016 to May 2018 were analyzed retrospectively. All patients were confirmed by conventional oral panoramic tomography examination and verified by surgery, and they were given MSCT and three-dimensional reconstruction due to ambiguous localization. The quantity, sites and shape of embedded teeth and root resorption and their relationship with the surrounding tissues were analyzed according to the images. Results A total of 84 embedded teeth were found among 76 patients. The number of single embedded teeth was the highest proportion (90.79%) in the total sum. The distribution of teeth sites was of higher proportion in maxillary canine (33.33%), central incisor (22.62%) and lateral incisor (17.86%). Labiopalatine lateral distribution was mainly embedded lingual side, accounting for 72.62%. The non-absorption of adjacent teeth accounted for 45.24%, and the light absorption accounted for 33.93%. The accuracy of MSCT was 100 % in the diagnosis of embedded teeth. Conclusion MSCT and three-dimensional reconstruction have accurate localization and diagnosis of embedded teeth, and it can clearly show the embedded teeth quantity, labiopalatine lateral distribution and root resorption. And it can be used as an important imaging reference for clinical correction.