Objective To explore the imaging features and clinical application value of glioma in dynamic enhanced MRI scan. Methods 39 patients with glioma admitted to our hospital from January 2013 to April 2018 were selected. All the patients underwent MRI examination, and the clinical data and imaging data were collected. The imaging features of 39 patients with glioma were analyzed and summarized by two associate chief physicians, and the clinical application value of dynamic enhanced MRI in glioma was analyzed. Results The main manifestations of glioma in MRI were long T1 and long T2 signals. Involvement range: 6 patients with 2 lobes and 3 lobes or greater were involved. Of the 39 patients, 21 were mainly involved in the temporal and frontal lobes. 16 patients with low-grade glioma presented low signal on T1WI, 12 presented high signal on T2WI, 9 presented equal signal, and 17 patients presented nodular enhancement in T1WI enhanced scanning. There were 16 cases of high-grade glioma with low signal on T1WI, 14 cases with high signal on T2WI, and 13 cases with edge enhancement in T1WI enhanced scan. The ADC value of low grade glioma was (1.23±0.26) s/mm2 , significantly lower than that of high grade glioma (0.86±0.14) s/mm2 , and the difference had statistical significance (t=5.397, P<0.001). Conclusion MRI dynamic enhanced scan can effectively display the signal characteristics and enhancement features of glioma, and multiple sequence scanning combined with ADC value can improve the accuracy of clinical evaluation of glioma staging and provide reliable data for clinical treatment planning.