Objective To investigate the clinical value of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and diffusion tensor imaging (DTI) in glioma grading. Methods A total of 105 cases of pathologically confirmed glioma patients in our hospital from January 2016 to December 2017 were selected, and all siemens received the DCE-MRI and DTI examinations. Results A total of 105 cases of pathologically confirmed glioma patients were consisted of 47 cases of astrocytoma (grade I), 35 cases of astrocytoma (grade II), 10 cases of ganglioneuroma at grade II, 3 cases of ependymoma at grade II, 6 cases of anaplastic astrocytoma (grade III), 4 cases of glioblastoma (grade VI). There were a total of 95 cases low-grade gliomas (I + II) and 10 cases high-grade gliomas (III+VI). The DTI values of brain tumoral parenchyma, peritumoral edema and outer of white matter region edema in high grade glioma group were significantly lower than those in low grade glioma group (P<0.05). The low grade glioma group had higher MD value and lower FA value at parenchyma edema than those of the high grade glioma group (P<0.05), while the low grade glioma group had higher MD value and lower FA value at normal tissues than those of the high grade glioma group, with no statistic difference (P>0.05). Conclusion High-grade gliomas have higher FA values and lower DTI and MD values. The imaging features are characterized as the disappeared distal fiber bundles, sparsely and partially interrupted tumor surrounding fiber bundles.