Objective To study the three-dimensional arterial spin labeling (3D-ASL) whole brain perfusion imaging in the diagnosis of brain tumor and application value of classification. Methods Retrospective analysis confirmed by histopathological and immunhistochemical of 40 cases of brain tumors, including 14 cases of gliomas (6 cases with low grade gliomas and 8 high grade glioma), 6 cases of myeloid sarcoma, 10 cases of meningioma and metastatic tumor, respectively. Measure 3D-ASL cerebral blood flow perfusion image tumor substantial part of the largest value (TBFmax), the contralateral normal white matter of cerebral blood flow (CBF) value and the relative tumor blood flow(rTBF). rTBF=TBFmax/CBF. Glioma were divided into low and high grade, meningioma, metastatic tumor group and myeloid sarcoma group, using the single factor analysis of variance of rCBF value comparison between groups, using the snk-q test to compare two difference was statistically significant (P<0.05). Results The rCBF of 3D-ASL was obtained quantitative magnetic resonance perfusion imaging parameters, quantitative rTBF myeloid sarcoma group were lower than low grade glioma group, the high grade glioma group, metastatic tumors and meningiomas group, the differences were statistically significant, rTBF low grade glioma group were lower than the group of high grade glioma, meningioma and metastases group, the differences were statistically significant, high grade glioma, meningioma,and among metastases was no statistically significant difference. Conclusion 3D-ASL can quantitatively reflect the perfusion of the tumor microcirculation, diagnosis, differential diagnosis and classification of brain tumors has important value.