Objective To investigate the differnce in clinical diagnostic value of multislice spiral CT and MRI in patients with biliary obstructive diseases. Methods 112 patients with biliary obstructive diseasesconfirmed by operative and pathologicalexamination were selected as the research objects.All the patients were examined by multi-slice spiral CT and MRI, and the results were recorded in detail. The results of operative and pathologicalexamination were taken as the gold standard to evaluate the differences in location and the accordance rate of qualitative diagnosis ofmulti-slice spiral CT and MRI. Results ①The coincidence rate ofmulti-slice spiral CT in lesionlocation was 95.5%. Compared with 97.3% of MRI, the difference was not statistically significant（P>0.05）. ②The accuracy rate of MRI in qualitative diagnosis ofbiliary obstructive diseases was 91.1% which was significantly higher than 77.7% ofmulti-slice spiral CT, and the difference was statistically significant (P<0.05). Conclusion To apply multiple-sequence MRI in diagnosis of patients with suspected biliary obstructiv diseases, the accuracy rate of location and qualitative diagnosis is relatively higher, which is beneficial to improve the accuracy and effectiveness of early diagnosis and has positive on saving consultation hours and carrying out the follow-uptreatmentsmoothly. It is worthy of clinical promotion.