Objective To investigate the clinical application value of color Doppler ultrasound and CT guided percutaneous lung biopsy in diagnosis of peripheral pulmonary masses. Methods 89 patients with peripheral lung masses in our hospital were divided into color ultrasound guidance group (n=51) and CT guided group (n=38). Guided percutaneous lung biopsy guided by color Doppler ultrasound guided percutaneous lung biopsy guided by CT guided percutaneous needle biopsy, two groups of patients were compared with groups of patients with one-time success rate, one-time success rate of one-time biopsy, pathological diagnosis rate and the incidence of complications. Results All the 89 cases were successful. Ultrasound guided group locate a one-time success rate 84.31% lower than in CT guided group of 97.37%. The difference between the two groups has statistical significance (χ2 =4.00, P<0.05); Ultrasound guided group disposable biopsy success rate was 96.08% higher than that of CT guided group of 92.11%, the difference between the two groups no statistical significance (χ2 =0.00, P>0.05); Ultrasound guided group biopsy pathological examination in the diagnosis of clear rate was 96.08% higher than that of CT guided group of 94.74%, the difference between the two groups with statistical significance (χ2 =0.07, P>0.05); Two groups there were no hem thorax, pin tract transfer, respiratory failure and other serious complications, the ultrasound guided group, the complication rate is 11.76% lower than in CT guided group 13.16% of the, the difference between the two groups no statistical significance (χ2 =0.02, P>0.05). Conclusion Color Doppler ultrasound and CT guided percutaneous lung biopsy is a safe, effective and feasible clinical diagnostic method, the ultrasound can display the pulmonary mass, recommended for the first choice of ultrasound guided biopsy, and the lung mass is selected CT guided biopsy.