Objective To investigate the MSCT features and clinical analysis of Warthin tumor in parotid gland, so as to improve the diagnostic accuracy. Methods Twenty-nine cases with parotid Warthin tumor with MSCT scanning were analyzed retrospectively, which were confirmed by operation and histopathology. The diagnostic accuracy of MSCT was compared with fine needle biography. Results In all of the 29 (male 25) cases, 22 cases were solitary lesions in unilateral, 2 cases were solitary lesions in bilateral, and 5 cases were multiple lesions, in which 4 cases were unilateral. Of all 45 lesions, 75.6% (34/45) lesions were located in the posterior and inferior quadrant. Four cases were nonenhancement MSCT scan, 25 cases were enhancement MSCT scan, 6 cases showed light enhancement, 12 cases median enhancement, and 23 cases showed prominent enhancement in arterial phase. 22 cases were diagnosed of Warthin tumor. A total of 14 cases were performed with fine needle aspiration cytology, 4 cases was not diagnosed with Warthin tumor. Conclusion The diagnostic value of MSCT in Warthin tumor of parotid gland was similar with fine needle aspiration cytology. Contrast-enhanced CT scan providing sufficient information of Warthin tumor in parotid gland, is useful in preoperation evaluation.