Objective Analyze the CT machine contaminated of methicillin-resistant staphylococcus aureus (MRSA) in radiological department to draw up the comprehensive and practical interventions. Methods The samples of the CT machine and Susceptible area of MRSA were collected from January to March 2015, afterwards, the bacterial contamination was detected. The12 weeks was separated into two stages that the former 6 weeks (the control groups) and the latter 6 weeks (the experimental groups).The samples of the control groups were collected after 2 hours of conventional sterilization. The samples of the experimental groups were collected after 2 hours sterilization with TCCA disinfectant ( chlorine 500 mg / L) and the related special interventions. Applying the same approach to sampling and the samples were collected to confirm MRSA strains. Results The difference of positive results of MRSA samples of two groups CT-related equipment has statistical significance (P＜0.05). The difference of two groups positive results of CT laboratory MRSA susceptible area samples has no statistical significance (P＞0.05). The positive rate of MRSA of the control groups was 16.67% ,and the the experimental groups was 3.03%. The difference of two groups has statistical significance (P＜0.05). Conclusions The most easily contaminated area by MRSA of CT laboratory is the direct contact area with patients. Using the comprehensive interventions enacted by ours research obtains a promising effect.It is illustrated that the comprehensive interventions can effectively reduce the infection rate .