Objective Comparative study of 64 slice CT perfusion imaging in solitary pulmonary nodules (SPN) and clinical application value in diagnosis of benign andmalignant. Methods For November to 2013 November in our hospital in 2011 50 cases of untreated diameter ≤ 3cm in solitary pulmonary nodules based on the pathological findings were divided into malignant nodules group andbenign SPNs group, respectively with 64 slice spiral CT perfusion imaging, bloodflowcalculation nodules (BF), bloodvolume (BV), theaverage pass time (MTT) and permeability value (PS). At the same time,rendering the same level nodules of time density curve (TDC),differentiating the benign and malignant nodules, and operation and pathology results were compared and analyzed, calculated the perfusion parameters for accuracy, diagnosis of malignant solitary pulmonarynodules sensitivity and specificity. Results He CT guided lung biopsy or operation and confirmed by pathology,found 34 cases of malignant nodules, 16 cases with benign nodules. Theperfusion parameters between two groups nodule significantly, P<0.01;TDC curve level with the benign and malignant nodules differ significantly, helps to prompt nodules (BV), blood volume, mean transit time (MTT), blood flow (BF) and permeability values (PS) for benign and malignant solitary pulmonary nodules, accuracy diagnostic sensitivityandspecificitywere 88%, 88.24%, 87.50%; 90%, 94.12%, 81.25%; 94%,91.12%, 100%; 86%, 94.12%, 68.75%. Conclusion 64 slice CT perfusion imaging is helpful to early diagnosis of solitary pulmonary nodules, provide ideas for further treatment.