Objective To investigate the effect of echo time, age, gender and the difference between right and left on metabolite quantity in front lobe and basal ganglia of MR spectroscopy in healthy adults. Methods MRS were employed in 17 right-handed healthy volunteers (5 male and 12 female, age range 20~60years, average age 48.5±16.5) used multi-voxel PRESS sequence with diffent TE(30ms and 135ms).Voxels were selected at the same areas of bilateral frontal white matter and basal ganglia, N-acetylaspartate(NAA), choline-containing compounds(Cho) and creatine(Cr) and NAA/Cr Cho/Cr were measured. And compared each statistical index between right and left, male and female. Results (1) We could got higher quality imagingsused PRESS135.And the NAA/Cr ratios in the frontal white matter and basal ganglia using PRESS135 and PRESS35 sequence had significant difference. However, there had no difference between them about the concentrations of Cho/ Cr in the basal ganglia. (2) The NAA/Cr had significant difference between right and left frontal white matter at the same TEs. However, no significant gender effects were found in the concentrations and their ratios. (3)Significant negative-correlation between age and NAA/Cr ratios were observed in the bilateral frontal white matter and right basal ganglia area. Conclusion This study demonstrated that different echo time, age laterality differences may affect the metabolicratios measured by MR spectroscopy, but there had no gender differences in both side of frontal white matter and right basal ganglia.