Objective 3D CT reconstruction technique was used to explore healthy adults lateral ventricle puncture of frontal horn of coronal plane of the width of the lateral ventricles of normal value range, to explore the most appropriate direction of frontal horn of lateral ventricle puncture. Methods The data of 68 cases of normal healthy adults in our hospital from January 2013 to January 2016 were collected, and the brain CT data of physical examination were collected. The groups were divided according to the age: age<44 group, (2)45<age<59 group,="" (3)60<age<74="" age="">75 group. 3D CT reconstruction technique was used on physical examination of frontal horn of lateral ventricle puncture in the coronal plane were reconstructed, ventricle width were measured in the plane of unilateral. 2.5 cm next to middle lane was used as standard puncture point, and then simulate the frontal horn of lateral ventricle puncture. Results The average width of the lateral ventricles in 68 healthy adults was (15.59+2.85)mm (95% confidence interval: 15.32 to 16.26mm). Age < 44 group of lateral ventricle width was (13.49 3.32mm), the average width of 45 < age < 59 group was(15.24+3.65)mm, average width of 60 < age < 74 group was (15.96+3.57mm), and the average width of age>75 group was (18.63+2.96)mm, the difference was statistically significant (P<0.05). All persons of average maximum partial open angle was (17.66+1.53), the statistical analysis found that the largest partial open angle was not statistically significant different in all age group (P>0.05), average minimum deviation angle was (60.8+3.27), and there were significant differences between the groups (P<0.05). Conclusion The width of the lateral ventricles healthy adult population will increases with the growth of the age, and partial angle will decrease with the growth of the age, the research found that the most suitable partial angle range of frontal horn of lateral ventricle puncture is between 6.08 to 17.66, can safeguard the success rate of the puncture.