Objective To investigate the medial temporal lobe perfusion changes in patients with temporal lobe epilepsy (TLE) in interictal period using dynamic susceptibility contrast enhanced (DSC) perfusion imaging. Methods 15 patients with pathologically confirmed right-handed TLE and matched 15 cases normal control were underwent DSC examination. Reconstructed the relative cerebral blood volume (rCBV) and relative cerebral blood flow (rCBF) using the workstation software, selected on both sides of the medial temporal lobe as region of interest combinedwith high-resolution anatomical image, then calculated the rCBV values, rCBF values and asymmetry index (AI), and compared the value between the the TLE group and the control group. Results 1. The rCBV and rCBF values in medial temporal lobe in normal control group showed no significant difference (P>0.05). 2. The rCBV and rCBF values in medial temporal lobe in affected side were lower than opposite side in TLE group, the difference was statistically significant (P<0.05). 3. The AIrCBV, AIrCBF in TLE group were higher than the normal control group, the difference was statistically significant (P<0.05). 4. AIrCBF and AIrCBV values in medial temporal lobe of TLE patients were positively correlated with disease course. Conclusion The rCBV and rCBF values were decreased in medial temporal lobe in patients with TLE in interictal period，the blood perfusion changes correlated with disease course in TLE patients，The longer the duration, the higher the perfusion asymmetry.