Objective To evaluate movement and morphologic change of the meniscal at different flexion angle of the knee by performing MRI scanning in human subjects, so as to obtain accurate and comprehensive data and the rule of the individual variation. Methods Thirty-four healthy knees from volunteers were examined by MRI,The knees were imaged with the knee flexed in different angle (0°, 30°, 60°) with a 3-dimemsional multil-data imaging being processed, the meniscal migration and height change were measured. Results The migration and the height change of the meniscus increased with the knee flexed. Posterior motion of lateral meniscus was greater than medial meniscus mostly, greater in the anterior horn than in the posterior horn (P<0.05). Both of the anterior and the posterior horns of the meniscus migrated posteriorly while the middle portion of the meniscus migrated laterally. There was a decrease in the migration from the 1st to 3rd, but negative values wereobserved from 4th to 7th with the direction being away from the meniscus. Conclusion It is a simple and non-invasive way that MRI scan of the knee at different degrees of flexion, demonstrating the kinematics and morphologic changes of the meniscus, providing better understanding of the meniscus diseases andobjective evidence for meniscus transplantation.