Objective To observe the influence of Metformin in adiponectin, total oxidation and insulin resistance of patients with PCOS and abnormal glucose metabolism. Methods A total of 112 cases of patients with PCOS and abnormal glucose metabolism were divided into observation group and contrast group randomly with each group 56 cases. Patients in both groups were given desogestrel and ethinylestradiol tablets treatment. Meantime, patients in the observation group were given Metformin based on desogestrel and ethinylestradiol tablets treatment. Observe and compare the APN level, insulin index as well as the levels of NO, TAC, MDA and SOD between both groups. Results The APN levels of after treatment in both groups were more raised than those of before treatment. In the contrast group, the difference of APN levels of before and after treatment showed no statistical significance (P>0.05). The APN levels in the observation group were more significantly raised than those in the contrast group. The difference showed s statistical significance (P<0.05). In the contrast group, the HOMA-IR of after treatment was not significantly different with that of before treatment. The difference showed s statistical significance (P<0.05). In the contrast group, the levels of NO, TAC, MDA and SOD of after treatment were not significantly different with those of before treatment (P>0.05). While in the observation group, the levels of NO and MDA were significantly more decreased and the levels of TAC and SOD were significantly more increased. The difference showed s statistical significance(P<0.05). Conclusion Metformin can increase the APN level of the patients with PCOS and abnormal glucose metabolism, improve the insulin resistance and total oxidation, and then promote to relieve the patients’ state of illness.