Objective To study the value of the observationnursing in operating room nursing. Methods A total of 48 patients in the operating room of our hospital from January 2015 to February 2016 were selected as the research object, and the 48 patients were divided into two groups at random, observation group and control group, control group patients took routine nursing, observation group took evidence-base nursing on the basis of control gruop, compared the satisfaction with nursing care of the patients, the surgery physician, and the anesthesiologist, and the intraoperative complications. Results The patient satisfaction of the observation group was 95.83%, the satisfaction of surgery doctors' was 100%, and the satisfaction of anesthesiologists was 100%, higher than the control group(P<0.01), the patient satisfaction in the control group was 66.67%, the satisfaction of surgery doctors' was 75%, and the satisfaction of anesthesiologists was 75%The complication rate of observation group was 4.17%,the control group was 29.17%, much higher than the observation group (P<0.01) with statistical significance. Conclusion Evidence-base nursing can increase the operating room nursing satisfaction of patients, and surgery physicians obviously, and reduce the incidence of intraoperative complications, and which deserve the clinical promotion.