Objective To investigate the oral Helicobacter pylori (HP) and its related impact on the eradication of Helicobacter pylori infection and treatment of Helicobacter pylori infection. Methods From June 2015 to May 2016, 50 cases of upper gastrointestinal symptoms in patients with newly diagnosed as group A in our hospital, select the radical mastectomy for 4 weeks were set as B group and 50 cases of gastric HP infection and 40 healthy persons as control group, all staff have to detect oral HP infection, the detection results of the three groups were compared. Results HPS detection of oral HP positive results: A group of 38 cases, the positive rate was 76%; B group in 37 cases, the positive rate was 74%, 40 patients in the control group, the positive rate of 80.0%. test results of three groups were two two compared to no difference, P>0.05. take 14C-UBT detection of gastric HP positive results: the positive rate of group A was 54%, the positive rate of B group was 34%, the positive rate of the control group Was 20.0%. Take 14C-UBT detection, a group of patients with gastric HP positive rate and B group, the control group is significantly higher, P<0.05. Take 14C-UBT detection of gastric HP positive patients, oral detection of HPS positive: a group, the positive rate was 88.9%. B group, the positive rate was 94.1%, the control group, the positive rate was 100.0%. Three groups of patients with H. pylori gastric HP positive patients without HPS positive detection rate of contrast difference, P>0.05. Conclusion Oral populations HP detection levels were higher, which may be important factors cause stomach-related diseases.