Objective To compare clinical application value between the 128-slice CT angiography and DSA angiography in the diagnosis of cardiovascular disease. Methods Clinical data of 40 patients with coronary heart disease from January 2013 to December 2014 were retrospectively analyzed. 128-slice CT coronary angiography and DSA angiography were underwent among those the patients after admission to hospital, and to compare the two methods in patients with coronary stenosis detection rate and spiral CT for coronary stenosis detection method sensitivity and specificity. Results The two methods in detecting severe coronary stenosis detection rate were close, the difference was not statistically significant (p> 0.05). Application of spiral CT coronary angiography in detecting severe coronary artery stenosis (coronary luminal diameter stenosis greater than 50%) misdiagnosed 11, missed 4, detection sensitivity of 88.17% and specificity of 92.41%. Conclusion The 128-slice CT coronary imaging technique is noninvasive detection methods which can greatly improve the detection rate of clinically suspected coronary heart disease, coronary artery disease with high detection sensitivity and specificity, because its costs are relatively non-invasive lower characteristics can be widely used in clinical.