目的 优化干扰素诱导的跨膜蛋白（Interferon induced transmenmbrane proteins,IFITMs）的五个亚型基因，即 IFITM1、IFITM2、IFITM3、IFITM5和IFITM10。方法 提取呼吸道感染样本中核酸，通过RT-qPCR筛选出具有特异性 的引物且Ct值在[15,35]之间，再利用琼脂糖凝胶电泳对引物的特异性进行近一步的验证。结果 RT-qPCR结果显示 IFITM1相关引物中IFITM1-3具有特异性；IFITM2相关引物中IFITM 2-2具有特异性；IFITM3相关引物IFITM3-2有较 好特异性；IFITM5相关引物中IFITM5-4具有特异性；IFITM10相关引物中IFITM10-1、IFITM10-2、IFITM10-3具有特 异性。具有特异性的所有引物的琼脂糖凝胶电泳条带单一明亮。结论 IFITM1-3优于该基因的其它引物；IFITM2-2 优于IFITM2-1；IFITM3-2优于IFITM3-1；IFITM10-1、 IFITM10-2 和IFITM10-3均适用。

Objective To optimize the five subtypes of interferon inducible transmembrane proteins, namely IFITM1, IFITM2, IFITM3, IFITM5 and IFITM10. Methods Extraction of nucleic acids from respiratory tract infection samples. Specific primers were screened by qRT-PCR and Ct value was between (15,35), and the specificity of primers was verified by agarose gel electrophoresis. Results IFITM1-3 was specific in the IFITM1 primers. IFITM 2-2 was specific in the IFITM2 primers. IFITM3-2 was specific in the IFITM3 primers. IFITM5-6 was specific in the IFITM5 primers. IFITM10-1, IFITM10-2 and IFITM10-3 were specific in the IFITM10 primers. Agarose gel electrophoresis bands of all primers with specificity were single bright. Conclusion IFITM1-3 was superior to other primers of IFITM1 primers. IFITM 2-2 was superior to other primers of IFITM2 primers. IFITM3-2 was superior to other primers of IFITM3 primers. IFITM5-4 was superior to other primers of IFITM5 primers. IFITM10-1, IFITM10-2 and IFITM10-3 were superior to other primers of IFITM10 primers.