Objective We present our initial experience with laparoendoscopic single-site surgery(LESS) by three-channel single port through a trans-umbilical access for adnexal tumors and report on the safety and feasibility of this technique. Methods A retrospective analysis was performed of 4 patients from October 2017 to December 2017 who underwent adnexal surgeries through LESS procedure by three-channel single port combined with the conventional laparoscopic instruments.LESS was performed by using a single port with triple-channel inserted method through a solitary 2.0 cm upper umbilicus incision and conventional laparoscopic instruments. Results All of the 4 patients had LESS surgeries successfully. No additional trocar required, no conversion to laparotomy happened. The operation incision scars of the 4 patients all healed well and were not obvious. The patients were discharged within 4-5 days after procedure without complications such as post-operative fever, umbilical hernia, hemorrhage or infection. Conclusion (1)With the strict precondition of indication, LESS using single port with triple-channel inserted method and conventional laparoscopic instruments can be a promisingly safe, minimally invasive and cosmetic approach to adnexal surgery.(2) Such technique can be used in therapy for pelvic endometriosis or ovarian endometriosis cyst, which may be more difficult.