Head and Neck Imaging
Effect of Vancomycin on SarA-mediated Biofilm Formation in MRSA
Author：WANG Fu-xiang.
affiliation：Department of Clinical Laboratory, the First Affiliated Hospital of Nanyang Medical College, Nanyang 473000, Henan Province, China
PDFAbstract
Objective Evaluation of the effect of vancomycin on SarA gene expression and biofilm formation in MRSA. Methods MERS strain 957 was collected from the blood cultures of patients with MRSA endocarditis in this hospital. A series of drug resistant strains 957-V8, 957-V16 and 957-V32 were obtained by culturing the mother strain with 957. Then the fluorescent gene quantitative assay was used to detect SarA mRNA levels in these four MRSA strains. The biofilm formation assay was performed using crystal violet staining. The absorbance of biofilm after staining was measured at 570nm. The tests were repeated 3 times. Results The SarA mRNA levels of 957-V8, 957-V16 and 957- V32 strains obtained in vitro were significantly higher than those of the original 957 strain.With the increase of MIC of vancomycin, the mRNA of SarA gene was gradually increased. All four strains can form biofilms, and the optical densities of induced strains 957-V8, 9527-V16 and 957-V32 biofilms were significantly higher than those of the original strain 957. With the increase of MIC value, the optical density of strain biofilm gradually increased. Conclusion Vancomycin can induce the increase of drug resistance and biofilm formation of Staphylococcus aureus, which is beneficial to the long-term existence of bacteria in vivo.
【Keyword】Vancomycin; MRSA; SarA; Biofilm
【Chart number】R318.021
【Document Identification Number】A
【DOI】10.3969/j.issn.1009-3257.2019.02.031
Journal of Rare Diseases
th26Volume， th 2 Issue
2019Year03Month
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