Objective To investigate the application value of multi-slice spiral CT perfusion imaging in renal clear cell carcinoma. Methods A total of 124 patients with renal clear cell carcinoma treated in our hospital from February 2015 to March 2017 were selected (observation group), and 120 healthy volunteers were selected as control group, multislice spiral CT perfusion imaging was performed to analyze the blood flow (BF), blood volume and aortic blood volume ratio (BV/A), mean transit time (MTT) and permeability surface (PS) in the areas of interest. Results The observation group BV/A, BF and PS were (0.16±0.03), (324.53±90.15) mL/min-1·100g-1 and (45.52±16.54) mL/min1 ·100g-1, significantly lower than the control group (P<0.05). The observation group and the control group MTT had no significant difference (P>0.05). The observation group BV/A and PS in patients with III-IV stage were (0.29±0.07) and (60.13±12.25) mL/min-1·100g-1, were significantly higher than that of patients with stage I and II (P < 0.05). BF in patients with III-IV stage and II stage were (361.51±92.22) mL/min1 ·100g-1 and (387.22±84.16) mL/min-1·100g-1, significantly higher than the patients with stage I (P<0.05). There were no statistically significant differences in MTT between different Robson stages(P>0.05). There were no significant difference in CT perfusion parameters between different age and genders of observation group (P>0.05). Conclusion Multi-slice spiral CT perfusion imaging has a certain value in the diagnosis of renal clear cell carcinoma and it can provide the basis for clinical staging.