Objective Comparative analysis of the effect of minocycline on cerebral ischemia -reperfusion in vivo rabbits by 64 - row CTP technique. Methods Fifty male New Zealand white rabbits were randomly divided into experimental group and control group, the rats in the experimental group were treated with minocycline before and after ischemia and reperfusion, and to analyze the difference of cerebral blood flow perfusion, cerebral blood volume, blood flow perfusion peak time and functional image difference and TTC staining in cerebral ischemia reperfusion group and control group. Results There was no significant difference between the CBF map and the defect area of the MTF map (P>0.05). There was no significant difference between the defective area of CVB and the defect area of TTB staining in different time periods (P<0.05). There was no significant difference between the two groups (P>0.05). There was no significant difference between the two groups (P>0.05). There was no significant difference between the two groups (P>0.05) No significant difference (P>0.05). The cerebral ischemic volume of the rabbits after cerebral ischemia and reperfusion was decreased and the significance was statistically significant (P<0.05). Conclusion Minocycline can improve the damage caused by ischemiareperfusion, and use CTP imaging to evaluate the accuracy and reliability of living rabbit blood flow changes. Therefore, 64-row CTP observation of minocycline can improve the cerebral ischemia-reperfusion in vivo, accurate, and worthy of clinical practice.