Objective To investigate the applicated value of dynamic contrast-enhanced MRI(DCE-MRI) in evaluating anti-angiogenic effect of Chinese medicine KLT in human colon cancer transplanted subcutaneously in nude mice. Methods 48 human colon cancer models of HT-29 which made by nude mice were established, and divided into experimental group and control group randomly, peritoneal cavities of the experimental group were injected KLT with dose 0.5mg/20g per day, and the control group were injected with the same volumes of saline in the same locations, DCE-MRI were performed before therapy and 7 days, 14 days, 21 days after treatment. 8 mouses were sacrificed respectively after MRI scanning in three time points, and pathological immunohistochemical examination were done. The following parameters(Ktrans, Kep,Veand Vp) which reflecting the tumor blood vessel functions were observed dynamically. The correlations between the changes of tumor tissue MVD and parameter values at each time point were analyzed. Results Ktrans and Kep of the experimental group decline and the control group rise gradually with the extended of time, the statistical differences in the two groups were existed(P＜0.05).There were no statistically difference between Ktrans and Kep of the two groups before treatments, however, Ktrans and Kep of the experimental group were obviously lower than the control group at each time point after treatments which were statistically significances(P＜0.05). Ktrans and Kep of the two groups after treatments were positively correlated with corresponded MVD at each time point(P ＜0.05). Conclusion KLT could inhibit the development of human colon cancer xenograft significantly, the dynamic changes of Ktrans and Kep values of DCE-MRI technology can be considered as a quantitative indexes for evaluating early colon cancer chemotherapy.