Objective To analyze the value of 320 slice volume CT one-stop scan in evaluating the prognosis of patients with liver cancer after transhepatic arterial chemotherapy and embolization (TACE). Methods 60 cases of patients with liver cancer confirmed by serum alpha fetoprotein (AFP), ultrasound and CT or puncture pathology were selected and were randomized into 2 groups. Before operation, CT plain scan and 320 slice CT one-stop scan were performed. With DSA as the golden standard, the accuracy of CT diagnosis was compared. In one week, TACE was performed and after operation, CT was performed. For patients with unsatisfying curative effect, they were treated with TACE and radiofrequency ablation (RFA). With 2 years of follow-up, the times of TACE and RFA and long-term survival rate were statistically analyzed. The guiding value of 320 slice volume CT one-stop scan in evaluating prognosis of patients with liver cancer after TACE was summarized. Results Before TACE, HAP and HAPI in liver cancerous tissues were higher than those in non-cancerous tissues while PVP was lower than that in non-cancerous tissues (P<0.05). After TACE, HAP and PVP increased while HAPI decreased. Compared with those in non-cancerous tissues, HAP and HAPI increased and PVP decreased (P<0.05). The display rates for grade 4 and grade ≥5 in group A were lower than those in group B and DSA. The display rate for grade ≥5 in group B was lower than that of DSA (P<0.05). DLP and ED in group A were lower than those in group B (P<0.05); The times of TACE and RFA again in group A was lower than that in B while the survival rate was higher than that in group B (P<0.05). Conclusions To adopt 320 slice volume CT scan before and after TACE can analyze the changes of perfusion parameters, dynamically evaluate curative effect, guide followed treatment, reduce the times of repeated treatment.