Objective To magnetic resonance imaging and ultrasonography in fetal agenesis of the corpus callosum screening diagnosis, analysis of the effect of diagnosis and imaging features. Methods By January 2011 to January 2015, 30 cases after the Institute of ultrasound and MRI diagnosis of corpus callosum of fetal development as the object, the medical records of all patients were retrospectively analyzed, divided into groups according to different methods to check will only accept ultrasonography of 15 cases of corpus callosum development whole fetuses divided to control group, will accept ultrasound combined with magnetic resonance imaging (MRI) of 15 patients with agenesis of the corpus callosum fetal divided to experiment group, two groups of corpus callosum body development not the fetal diagnosis results and follow-up results compared. Comparing the two kinds of inspection methods of diagnosis accurate rate. At the same time on the development of fetal corpus callosum ultrasound imaging insufficiency and magnetic resonance imaging analysis. Results The experimental group of fetal agenesis of the corpus callosum in 10 cases, 5 cases of complete agenesis of the corpus callosum, as part of the corpus callosum agenesis. The accuracy of diagnostic ultrasound and magnetic resonance of the experimental group was 93.3% (14/15). The control group of fetal agenesis of the corpus callosum in 9 cases, 6 cases of complete agenesis of the corpus callosum, as part of the corpus callosum agenesis. The diagnostic accuracy rate of control group was 86.7% (13/15). The accurate rate of diagnosis showed the experimental group was significantly higher than that of control group, and the difference of P<0.05 between groups. Conclusions In prenatal fetal of ultrasonography and magnetic resonance imaging (MRI) can effectively improve the fetal corpus callosum body development is full of screening and diagnosis rate, the clinical diagnosis of fetal corpus callosum development has a very important significance.