Objective To analyze the hemodynamic changes by multi-slice spiral CT perfusion parameters of liver nodules, and to evaluate its diagnostic value. Methods 110 patients who were admitted in our liver and gall surgical department from January 2011 to June 2014 were selected as research objects. 60 cases with primary liver cancer were observation group, and 50 healthy person were control group. All subjects underwent CT perfusion scanning and enhanced scanning. The parameters includs HBF, HBV, HAP, HAI, HPP, MTT, and PS, and the differences were analyzed. Results There were no significant differences in HBF, HBV, HAP, HAI, HPP, MTT, and PS among the three groups (P <0.05). HBF, HAI, MTT, and HAP of HCC nodules group were higher than the control group, and PS was lower than the control group(P<0.05). HBV and HPP had no significant difference between HCC nodules group and the control group(P> 0.05); HBF, HAP, HAI, MTT, and HPP of HCC nodules group were higher than peritumor group, and the difference was statistically significant (P<0.05); HBV and PS had no significant difference between HCC nodules group and peritumor group(P> 0.05). HAP, PS, MTT, and HPP had significant difference (P<0.05) and HBF, HBV, and HAI had no significant difference(P>0.05) between peritumor group and the control group. Conclusion Multi-slice CT has well resolution in the detection of blood dynamic changes of liver nodules, which can effectively identify different types of tissue perfusion and improve the sensitivity and specificity of the imaging diagnosis of liver nodules.